| Application | For immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host. |
| | Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli. |
| Features and Benefits | ANTI-FLAG M5 − Biotin conjugate has a high affinity for N-terminal Met-FLAG fusion proteins. |
| Other Notes | To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature. |
| Physical form | Solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 0.02% sodium azide |
| Legal Information | ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| | FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
